Question

Please read the paragraph below to answer the following questions.
You conducted a series of experiments to test whether the expression of hexokinase, an important enzyme involved in glycolysis, varies under different conditions. In the first experiment, you measured the production of hexokinase mRNA in cells at three different temperatures: 37ºC, 25ºC, and 10ºC. Your results showed that the mRNA levels for hexokinase were 5 and 20 lower at 25ºC and 10ºC than at 37ºC, respectively. Based on these results, you are going to conduct further experiments to attempt to answer your research question.
DNA microarrays can be used to determine relative levels of gene expression between samples. DNA microarrays are usually interpreted by color: green means higher mRNA levels, yellow means equal levels, and red means lower levels. If you ran the samples from the temperature experiment on a DNA microarray using the 25C experiment as a baseline, what results do you predict you would obtain?
A) The samples from the 37ºC experiment would be green and the samples from the 10ºC would be red.
B) The samples from the 37ºC experiment would be red and the samples from the 10ºC would be green.
C) The samples from the 37ºC experiment would be green and the samples from the 10ºC would be yellow.
D) The samples from the 37ºC experiment would be yellow and the samples from the 10ºC would be red.

Answer

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