Question

Please read the paragraph below to answer the following questions.
You conducted a series of experiments to test whether the expression of hexokinase, an important enzyme involved in glycolysis, varies under different conditions. In the first experiment, you measured the production of hexokinase mRNA in cells at three different temperatures: 37ºC, 25ºC, and 10ºC. Your results showed that the mRNA levels for hexokinase were 5 and 20 lower at 25ºC and 10ºC than at 37ºC, respectively. Based on these results, you are going to conduct further experiments to attempt to answer your research question.
What would be the best experiment to do next to determine if complete hexokinase gene expression is temperature dependent?
A) Measure the amount of hexokinase mRNA in cells at different temperatures.
B) Measure the rate of transcription of the hexokinase gene in cells at different temperatures.
C) Measure the amount of mRNA that is broken down in cells at different temperatures.
D) Measure the amount of hexokinase protein in cells at different temperatures.

Answer

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